Engineered microbes are of great potential utility in biotechnology and basic research. In principle, a cell can be built from scratch by assembling small molecule sets with auto‐catalytic properties. Alternatively, DNA can be isolated or directly synthesized and molded into a synthetic genome using existing genomic blueprints and molecular biology tools. Activating such a synthetic genome will yield a synthetic cell. Here we examine obstacles associated with this latter approach using a model system whereby a donor genome from H. influenzae (...) is fragmented, and the pieces are then modified and reassembled stepwise in an E. coli host cell. There are obstacles associated with this strategy related to DNA transfer, DNA replication, cross‐talk in gene regulation and compatibility of gene products between donor and host. Encouragingly, analysis of gene expression indicates widespread transcription of H. influenzae genes in E. coli, and analysis of gap locations in H. influenzae and other microbial genome assemblies reveals few genes routinely incompatible with E. coli. In conclusion, rebuilding and booting a genome remains a feasible and pragmatic approach to creating a synthetic microbial cell. BioEssays 29:580–590, 2007. © 2007 Wiley Periodicals, Inc. (shrink)

De Neys makes some useful points regarding dual-process models, but his critique ignores highly relevant theories of judgment from the persuasion literature. These persuasion models predate and often circumvent many of the criticisms he makes of the dual-process approaches he covers. Furthermore, the persuasion models anticipated some of the correctives to dual-process models that he proposes.

In this study, we comprehensively examine the relationships between ethical leadership, social exchange, and employee commitment. We find that organizational and supervisory ethical leadership are positively related to employee commitment to the organization and supervisor, respectively. We also find that different types of social exchange relationships mediate these relationships. Our results suggest that the application of a multifoci social exchange perspective to the context of ethical leadership is indeed useful: As hypothesized, within-foci effects (e.g., the relationship between organizational ethical leadership (...) and commitment to the organization) are stronger than cross-foci effects (e.g., the relationship between supervisory ethical leadership and commitment to the organization). In addition, in contrast to the “trickle down” model of ethical leadership (Mayer et al. in Org Behav Hum Decis Process 108:1–13, 2009), our results suggest that organizational ethical leadership is both directly and indirectly related to employee outcomes. (shrink)

Malebranche and the Search after Lost Identity. Legacy and Uncertainty of His Philosophy between the Eighteenth and Twentieth Centuries. Starting from the Œuvres complètes edited by Robinet, the article summarizes the vicissitudes through which the French historical scholarship with its leading figure Victor Cousin have restored the imagine and the correspondence of Malebranche, beginning with the dissemination of the original documents, conserved throughout the eighteenth century by P. Lelong, P. André, and finally P. Adry. In spite of his great historical (...) contribution, the French scholarship generally stressed an inadequate assessment of this philosophy, sharply suspected of spinozism, a judgment inherited by the handbooks of the history of philosophy edited between the XIX and the XX Century, as proved by the case of Grundriss der Geschichte der Philosophie by Überweg. The article concludes with three minimalist but creative readings of Malebranche by Proust, Heisenberg, Dalì. (shrink)

Il presente contributo muove dalle osservazioni sul linguaggio che Nietzsche svolge in Umano, troppo umano, I, § 11, con lo scopo di riflettere sulla posizione anti-realista che Nietzsche sostiene in quell’aforisma e di evidenziare il ruolo che essa svolge nelle sue più tarde considerazioni relative alla cultura occidentale e alla sua antropologia. Come si avrà modo di mostrare, la critica nietzscheana al realismo del senso comune è in linea con alcune epistemologie pragmatiste sorte tra Otto- e Novecento. Questo elemento di (...) “attualità” non mina però l’originalità della riflessione di Nietzsche su quell’argomento. Piuttosto, è possibile sostenere che l’idea che il pensiero filosofico intervenga in una correzione dell’impegno metafisico proprio del senso comune sia lo strumento teoretico che permette a Nietzsche di operare sullo sviluppo della cultura e società europee. (shrink)

The paper explores the few occurrences of the expression «will to nothingness» (Wille zum Nichts) in Nietzsche’s writings, and its relationship with the notions of ‘will to truth’ and ‘ascetic ideal’. Aim of this research is to show that these notions are mutually related, and that they outline the objectives of Nietzsche’s late thought. The investigation will focus in particular on the concept of “realism” that appears in Nietzsche’s late writings, and that can be interpreted as an existential attitude towards (...) life that contrasts pessimistic nihilism. (shrink)

As considerações de Merleau-Ponty sobre a historicidade são significativas, tanto como um relato fenomenológico, quanto como um relato existencial de nossa situação histórica. Naturalmente, estes aspectos de seu trabalho não se excluem mutuamente, mas se entrelaçam. E cada aspecto contribui para o sentido da crise da historicidade humana. Primeiro consideraremos a crise fenomenológica da historicidade – ou seja, consideraremos a crise da historicidade fenomenologicamente. Esta é a tentativa de revelar as condições da possibilidade da história a partir da história ou (...) da estrutura de nossa situação histórica – onde a posição de Merleau-Ponty se afasta da fenomenologia husserliana. Em seguida, consideraremos a crise existencial de historicidade – ou seja, consideraremos a crise de historicidade existencialmente. Esta é a tentativa de compreender o imperativo de que as coisas são importantes para nós historicamente. Finalmente, vamos indicar brevemente a importância destes aspectos de nossa historicidade como um entrelaçamento crítico. Este entrelaçamento crítico revela a intrusão mútua do “como” e do “porquê” de nossa historicidade, que Merleau-Ponty descreve no final de sua carreira como uma existência chiasmática dentro da carne do mundo. O cruzamento crítico como interrogatório de nosso ser histórico fornece o “quem”, “o quê”, “quando” e “onde” de nossa historicidade. (shrink)

«Questo è il vero fenomenalismo e prospettivismo, come lo intendo io», scrive Nietzsche in FW 354, chiudendo una lunga riflessione sul tema della coscienza e del bisogno di comunicazione dell’uomo. Mantenendo sullo sfondo le questioni più strettamente legate alla dimensione psicologica, vorrei partire da questa dichiarazione per considerare alcuni aspetti della teoria della conoscenza di Nietzsche ed intervenire in una nuova determinazione del suo carattere prospettico. In particolare, vorrei soffermarmi sul tema del gregge umano e della specie come reale soggetto (...) della visione prospettica, per poi discutere il riferimento al fenomenalismo rintracciabile in alcuni appunti dedicati ad una declinazione del Wille zur Macht come conoscenza. (shrink)

Robert Litman descreve quatro casos extraordinários em que indivíduos revelam esse conhecimento reprimido por meio da análise de sonhos após o suicídio do “outro”. Em cada caso, ocorre um momento de reconhecimento da significância do sonho tal que o sujeito se dá conta da culpabilidade. E, em cada caso, essa culpabilidade teve que ver com uma interrupção na comunicação que é revelada através da psicanálise. Quero desconstruir a relação transferência-contratransferência como uma reciprocidade simbiótica. Não ouso adiante, inalterado, quando transcendo quem (...) eu era através da língua com outros. A língua é a alteração da subjetividade. Ofereço uma crítica de certas compreensões de alteridade envolvidas em psicoterapia pelo exame da situação geral do encontro com outros por meio do evento-língua, peculiar à psicoterapia. Devo reconhecer que a ameaça da alteridade do paciente é também a promessa da minha habilidade em oferecer ajuda. Se consideramos seriamente a transcendência do evento-língua da terapia, vemos que o processo de alteração do outro implica mudanças para todos os envolvidos.Robert Litman describes four remarkable cases where individuals reveal this repressed knowledge through dream analysis after the suicide of the “other.” In each case, there comes a moment of recognition of the significance of the dream such that the subject realizes culpability. And in each case, this culpability had to do with a communication breakdown that is revealed through psychoanalysis. I want to deconstruct the transference / counter-transference relationship as a symbiotic reciprocity. I do not venture forth unchanged as I transcend who I was through language with others. Language is alteration of subjectivity. I offer a criticism of certain understandings of alterity involved in psychotherapy by examining the general situation of the encounter with others through the peculiar language-event of psychotherapy. I must recognize that the threat of the alterity of the patient is also the promise of my ability to offer help. If we take seriously the transcendence of the language-event of therapy, we see that the process of altering another involves changes for all involved. (shrink)

ITA: Quello del “buon europeo” è in Nietzsche un tema significativo, che si presenta originariamente connesso alle riflessioni di Nietzsche sulla cultura europea, arricchendosi col tempo di una portata filosofica che si lega agli obiettivi del suo pensiero maturo. Scopo del presente articolo è di mostrare la genesi e l’evoluzione di tale concetto, a partire dalle sue prime occorrenze in Umano, troppo umano I fino al suo compiuto sviluppo negli scritti del 1885-87. Tale studio permetterà di evidenziare il particolare valore (...) che Nietzsche attribuisce ai “buoni europei”, in quanto promotori dello sviluppo spirituale conseguente alla compiuta liberazione della morale cristiana. ENG: The “good European” is a rich and important topic in Nietzsche. It is first related to Nietzsche’s early reflections on European culture, and during the 1880’s it gains philosophical value, being strictly connected with the purposes of Nietzsche’s mature thought. The aim of this paper is to track the genesis and development of the notion of “good European”, from Human, all too Human I to Nietzsche’s writings from the years 1885-1887. That investigation shall particularly show the meaning and value that Nietzsche attributes to the good Europeans; as for him, they are in fact the leaders of the spiritual development that follows the overcoming of Christian morality. (shrink)

Aim of this paper is to reflect on the anthropological ideal Nietzsche outlines in his late period. The way Nietzsche deals with concepts such as “German”, “(good) European”, and “free spirit” in the sections of Twilight of the Idols which deal with Goethe will be especially considered. Furthermore, I shall argue that Renaissance plays an important role in Nietzsche’s anthropological project, for it helps to define the spiritual strength which characterizes the health type of man to which Nietzsche destined his (...) late philosophical wisdom and gay science. (shrink)

Tra i numerosi autori attivi nel campo delle scienze naturali che Friedrich Nietzsche ebbe modo di conoscere nel corso della propria vita, Ernst Mach rappresenta certamente un caso significativo. La sua presenza all'interno degli scritti del filosofo è pressoché nulla, ma la comunanza dei temi trattati e la particolare affinità delle prospettive adottate in materia di teoria della conoscenza invitano ad avvicinare questi due autori e a ipotizzare un qualche tipo di influsso diretto tra loro. Ciononostante, fino ad oggi non (...) era mai stato svolto uno studio critico e completo che esaminasse e discutesse tale relazione. Muovendo dai contenuti di una delle principali opere di Mach, i "Beiträge zur Analyse der Empfindungen" (1886) questo volume delinea un quadro esegetico che fa di Mach non tanto un referente diretto, quanto piuttosto il segnale di una prospettiva di ricerca che in qualche modo stimolò l'interesse di Nietzsche e che per molti aspetti egli mostra di condividere. (shrink)

Ernst Mach (1838-1916) è stato una figura di riferimento per la cultura scientifica e filosofica tardo-ottocentesca e dei primi decenni del Novecento. Le sue ricerche in fisica e psicologia, così come il lavoro epistemologico che emerge dalle pagine di opere quali La meccanica nel suo sviluppo storico-critico e Conoscenza ed errore, hanno influito notevolmente su molti autori a lui contemporanei. In questi testi, Mach delinea una concezione antimetafisica del pensiero scientifico e una concezione biologico-evolutiva della conoscenza umana che si ritrovano (...) elaborate in vario modo nella teoria della relatività di A. Einstein, nell’epistemologia evoluzionistica di K. Popper e D. Campbell, nel pragmatismo di W. James e, più in generale, nelle idee che animarono il primo Circolo di Vienna. I saggi raccolti nel presente volume si propongono di commemorarne la figura e l’opera, guardando a Mach come figura di confine tra prospettive di indagine che la storia della filosofia dell’ultimo secolo ha spesso visto contrapposte. (shrink)

During the second decade of the 20th century Hans Kleinpeter, an Austrian scholar devoted to the development of the modern science, published some brief papers on Nietzsche’s thought. Kleinpeter has been one of the main upholders of Mach’s epistemology and probably the first who connected his ideas with the philosophy of Nietzsche. In his book on Der Phänomenalismus (1913) he described a new world view that arose in the 19th century, a perspective that ‒ according to him ‒ completely contrasted (...) the mechanistic and metaphysical world view of the old school of scientific inquiry. The main outcome of the scientists whose name was related with this perspective (e.g. Clifford, Maxwell, Kirchoff and, obviously, Mach himself) has been the refusal of the absolute value of any “truth”. Kleinpeter’s statements on this topic are a good example of the rising of a Scientific Philosophy, whose development involved many scientists and thinkers that later set up the Verein Ernst Mach and the Wiener Kreis. On the other hand, his interest on Nietzsche is a relevant case of reception of the latter’s thought, that Kleinpeter puts into the context of the contemporary epistemology. In fact, he considers Nietzsche as one of the main upholders of the phenomenalistic world view, and states that he «took part at the same renewal of philosophical investigation that arose from the latest results of scientific inquiry» during the 19th century. A renewal whose main outcomes has been presented by John Stallo in his book on The Concepts and Theories of Modern Physics (1881), which Kleinpeter translated in German and published in 1901. According to Kleinpeter, in Nietzsche’s writings (mostly unpublished) one can find a theory of knowledge quite close to the one presented by both Mach and the new born Pragmatism, i.e. the complete refusal of an absolute truth and, therefore, the development of an antimetaphysical world view. In my paper I’ll discuss the main statements presented by Kleinpeter on this topic and show which of Nietzsche’s ideas has actually been in compliance with the main outcomes of late 19th century science. Thus, I’ll carry out a reconstruction of an unfamiliar side of the first period of reception of the philosophy of Nietzsche and its relevance to the development of a new (scientific) world view. (shrink)

Friedrich Nietzsche’s criticism towards the substance-concept „I“ plays an important role in his late thought, and can be properly understood by making reference to the 19th century debate on the scientific psychology. Friedrich Lange and Ernst Mach gave an important contribution to that debate. Both of them developed the ideas of Gustav Fechner, and thought about a „psychology without soul“, i.e. an investigation that gives up with the old metaphysics of substance in dealing with the mind-body problem. In this paper (...) I shall deal with both Lange and Mach (whose writings has been read by Nietzsche), in order to shed some light on Nietzsche’s rejection of the „I“ in philosophy. (shrink)

At the beginning of the twentieth century, theorists developed approaches to Nietzsche’s philosophy that provided an alternative to the received view, some of them suggesting that his view of truth may be his most important and original contribution. It has further been argued that Vaihinger’s fictionalism is the paradigm within which Nietzsche’s view can be properly contextualized. As will be shown, this idea is both viable and fruitful for solving certain interpretive issues raised in recent Nietzsche scholarship.

Il contenuto dei paragrafi conclusivi della "Genealogia della morale" può essere considerato il nucleo di una rete di concetti che costituisce l’intelaiatura della tarda filosofia di Nietzsche. In questo saggio si mostrerà in particolare come la problematizzazione della verità annunciata in GM III, 24 e 27 rappresenti per Nietzsche un passaggio fondamentale per portare a maturazione le istanze critiche del proprio pensiero e permettere la realizzazione della «filosofia dell’avvenire» di cui "Al di là del bene e del male" doveva costituire (...) il preludio. (shrink)

Nel XIII sec., il dibattito su Dio come primum cognitum si sviluppò all'interno della tradizione agostiniana anche attraverso la mediazione di Avicenna, per poi essere decisamente attaccato da Tommaso. La prima metà dello studio verte sulla posizione di Guiberto, esposta nel suo Rudimentum doctrinae, dove il problema è inquadrato all'interno della dottrina agostinana dell'illuminazione, e rapportato alla tesi bonaventuriana. La seconda parte è invece centrata sulla confutazione tomista, desunta principalmente dal prologo e dal trattato Super librum Boetii De trinitate. Nelle (...) conclusioni l'A. ribadisce, tra l'altro, la posizione intermedia presente nella Summa fratris Alexandri. (shrink)

Recent systematic studies using newly developed genomic approaches have revealed common mechanisms and principles that underpin the spatial organization of eukaryotic genomes and allow them to respond and adapt to diverse functional demands. Genomes harbor, interpret, and propagate genetic and epigenetic information, and the three‐dimensional (3D) organization of genomes in the nucleus should be intrinsically linked to their biological functions. However, our understanding of the mechanisms underlying both the topological organization of genomes and the various nuclear processes is still largely (...) incomplete. In this essay, we focus on the functional relevance as well as the biophysical properties of common organizational themes in genomes (e.g. looping, clustering, compartmentalization, and dynamics), and examine the interconnection between genome structure and function from this angle. Present evidence supports the idea that, in general, genome architecture reflects and influences genome function, and is relatively stable. However, the answer as to whether genome architecture is a hallmark of cell identity remains elusive. (shrink)